DNA
O5
Part:BBa_K2054005
Designed by: LAI HEI WAI Group: iGEM16_Hong_Kong_HKU (2016-10-14)
Oligo 5 of DNA Tetrahedral Nanostructure
This will be used to build our desired nanostructure that target micro RNA biomarkers. This plasmid encodes the second ssDNA that will make the tetrahedral nanostructure, together with oligo 1, 2, 3 and 4.
Usage and Biology
Characterization conducted by:
- Group: Team: HK_SKHLPSS, 2019
- Author: Tse Cheong Tat
- Summary: The peroxidase activity of the nano-structure formed from parts BBa_K2054001and BBa_K2054005 were measured. It was found that the nano-structure formed from these two parts had a high specificity in detecting the target sequence, showing that the purified ssDNAs produced from plasmid could work as per their design and function well. Details of our experimental characterization could be found on here.
- Relevant documentation:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Sequence and Features
Assembly Compatibility:
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
//dna
//dna
chassis | E. coli (DH10B) |